Hygromycin B, EvoPure^®solution (100 mg/mL in PBS Buffer) is a solution containing highly pure (>99.0%) Hygromycin B formulated in PBS buffer at a concentration of 100 mg/ml. Hygromycin B is a unique aminoglycoside antibiotic derived fromStreptomyces hygroscopicusand is routinely used as a selective agent in cell culture or microbiology applications to isolate Hygromycin B resistant cells after transfection or transformation, respectively.

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CAS Number

31282-04-9

Molecular Formula

C₂₀H₃₇N₃O₁₃

Molecular Weight

527.52 g/mol

Mechanism of Action

Hygromycin B inhibits protein synthesis by strengthening the interaction of tRNA binding in the ribosomal A-site. Hygromycin B also prevents mRNA and tRNA translocation by an unknown mechanism. These are unique mechanisms for an aminoglycoside antibiotic and they differ from the mode of action of Neomycin, Gentamicin, and G418.

Mechanism of Resistance

Hygromycin B resistance is conferred by thehphgene isolated fromStreptomyces hygroscopicus , a1467 bp fragment which encodes Hygromycin B phosphotransferase (HPh). Cell lines successfully transfected with thehphgene produce hygromycin B phosphotransferase and convert hygromycin B to 7”-O-phosphoryl-hygromycin B by phosphorylating the 4-hydroxyl group on the cyclitol ring of hygromycin B. 7”-O-phosphoryl-hygromycin B lacks antibiotic activity and does not interact with prokaryotic or eukaryotic ribosomes.

Storage Conditions

2-8 °C

Tariff Code

2941.90.1010

Spectrum

Hygromycin B is effective against eukaryotic and prokaryotic cells.