G418 Disulfate (syn: Geneticin; G418 sulfate) is routinely used for gene selection in cell culture. G418 Disulfate is an aminoglycoside antibiotic isolated from Micromonospora rhodorangea and closely related to Gentamicin.

We also offer:

• G418 Disulfate Solution (50 mg/ml) in Water (G020-G021)
• G418 Disulfate, EvoPure (G030)
• G418 Disulfate, (Low Endotoxin) (G048)

CAS Number

108321-42-2

Molecular Formula

C₂₀H₄₀N₄O₁₀ · 2H₂SO₄

Molecular Weight

692.71

Mechanism of Action

G418 Disulfate, and other aminoglycosides prevent protein synthesis. Resistance to G418 Disulfate is conferred by the neo gene (neomycin resistant gene) from either Tn5 or Tn601 (903) transposons. C ells successfully transfected with resistance plasmids containing the neo resistance gene can express aminoglycoside 3'-phosphotransferase (APT 3' I or APT 3' II) which covalently modifies G418 to 3-phosphoric G418, which has negligible potency and has low-affinity for prokaryotic and eukaryotic ribosomes.

Storage Conditions

Ambient

Tariff Code

2941.90.1010

Spectrum

G418 Disulfate is toxic to susceptible bacteria, fungi, yeast, protozoa, helminths, mammalian cells, and plants.