Methotrexate is a selective agent for dihydrofolate reductase (DHFR)- transfected cells, and a nucleic acid synthesis inhibitor in protein expression systems, thus can be used as an ancillary material in upstream biomanufacturing applications. It also has immunosuppressive effects for rheumatoid arthritis research and has anti-cancer properties. Methotrexate is soluble in DMSO but practically insoluble in water.
We also offer:
- Methotraxate sodium salt (M031)
- Methotrexate, EvoPure®(M091)
This product is considered a dangerous good. Quantities above 1 g may be subject to additional shipping fees. Please contact us for questions.
CAS Number
59-05-2
Molecular Formula
C20H22N8O5
Molecular Weight
454.44
Mechanism of Action
In cancerous cells, methotrexate acts as an allosteric inhibitor of dihydrofolate reductase (DHFR), an enzyme involved in the folic acid metabolic pathway, catalyzing the conversion of dihydrofolate to tetrahydrofolate. Since tetrahydrofolate is needed for synthesis of purine and thymidine synthesis, it results in the inhibition of DNA and RNA synthesis.
