HygromycinBisauniqueaminoglycosideantibioticderivedfromStreptomyceshygroscopicus. HygromycinBisroutinelyusedasaselectiveagentincellcultureormicroBIOLOGyapplicationstoisolatehygromycinBresistantcells.TOKU-Erecommendspreparingastocksolutionat50mg/mL-100mg/mL.TOKU-EalsooffershygromycinBsolutionconvenientlypreparedat50mg/mLinPBSbuffer.

Thisproductisconsideredadangerousgood.Quantitiesabove1gmaybesubjecttoadditionalshippingfees.Pleasecontactusforspecificquestions.

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CASNumber

31282-04-9

MolecularFormula

C₂₀H₃₇N₃O₁₃

MolecularWeight

527.52

MechanismofAction

HygromycinBinhibitsproteinsynthesisbystrengtheningtheinteractionoftRNAbindingintheribosomalA-site.HygromycinBalsopreventsmRNAandtRNAtranslocationbyanunknownmechanism.Theseareuniquemechanismsforanaminoglycosideantibioticandtheydifferfromthemodeofactionneomycin,gentamicin,andG418.

Mechanismofresistance:

HygromycinBresistanceisconferredbythehphgeneandisisolatedfromStreptomyceshygroscopicusDNA.ThehphhygromycinBresistancegenesequenceisa1467bpfragmentwhichencodeshygromycinBphosphotransferase(HPh).CelllinessuccessfullytransfectedwiththehphgeneproducehygromycinBphosphotransferaseandconverthygromycinBto7”-O-phosphoryl-hygromycinBbyphosphorylatingthe4-hydroxylgrouponthecyclitolringofhygromycinB.7”-O-phosphoryl-hygromycinBlacksantibioticactivityanddoesnotinteractwithprokaryoticoreukaryoticribosomes.

StorageConditions

2-8°C

TariffCode

2941.90.1010

Spectrum

HygromycinBiseffectiveagainsteukaryotic(mammalian)andprokaryotic(bacteria,fungi/yeast)cells.

Specifications:

Form

Powder

Appearance

Off-WhiteorLightTanPowder

Source

StreptomycesHygroscopicus.

WaterContent(KarlFisher)

≤15.0%

Potency(onadrybasis)

≥900u/mg

MeltingPoint

160-180°C

LossonDrying

≤5.0%