Form
Powder
Appearance
Whiteoroff-whitepowder
Source
Streptomycesgriseochromogenes
Potency(onadrybasis)
≥850µg/mg
Endotoxin
≤1.0EU/mg
BlasticidinSHClisanucleosideantibioticderivedfromStreptomycesgriseochromogenes.BlasticidinSHClisroutinelyusedincellculturegeneselectionapplicationsasaselectableagenttoisolateblasticidinSresistantmammalianandbacterialcells.
TOKU-Erecommendspreparingstocksolutionsat5-10mg/mLinwateror20mMHEPES.Stocksolutionscanbestoredat4°Cforshorttermstorageand-20°Cforlongtermstorage.
TOKU-Ealsosupplies:
Thisproductisconsideredadangerousgood.Quantitiesabove1gmaybesubjecttoadditionalshippingfees.Pleasecontactusforspecificquestions.
Luetal. usedblasticidinSHClandpuromycindihydrochloridefromTOKU-EtoselectfortransfectedAS-B145andBT-474cells. "OvatodiolideInhibitsBreastCancerStem/ProgenitorCellsthroughSMURF2-MediatedDownregulationofHsp27"
3513-03-9
C17H26N8O5·HCl
458.90
BlasticidinSHClinhibitsproteinsynthesisinprokaryoticandeukaryoticcellsbybindingtotheribosomalP-sitewhichstrengthenstRNAbindingandslowsdownandpreventssubsequentpeptidesynthesis.
ResistancetoblasticidinSisconferredbybsr,BSD,andblsresistancegenesisolatedfromBacilluscereusK55-S1,Aspergillusterreus,andStreptoverticillumspp,respectively.
Thebsrresistancegeneisa420bpfragmentandencodesa15kDablasticidinSdeaminasewhichcatalyzesthereactionofblasticidinStodeaminohydroxyblasticidinS.DeaminohydroxyblasticidinSisaBIOLOGicallyinactivederivativeofblasticidinSanddoesnotinteractwithorinhibitprokaryoticoreukaryoticribosomes.
Thebsdresistancegeneisa393bpfragmentandalsoencodesablasticidinSdeaminaseenzymewhichcatalyzesasimilarreactiontotheBSRdeaminase.AstudybyKimuraetal.foundthetransfectionfrequencywithbsdtobe80XgreaterthanwithbsrwhenusingFM3Acells.
Theblsgeneresistancegeneencodesanacetyltransferasewhichinteractswithacetyl-coenzymeAandpreventsblasticidinSfrominhibitingproteinsynthesis.
2-8°C
2934.99.9000
BlasticidinSHClisbiologicallyactiveagainstsusceptIBLemammalianandprokaryoticcells.
Powder
Whiteoroff-whitepowder
Streptomycesgriseochromogenes
≥850µg/mg
≤1.0EU/mg