Methotrexate is a selective agent for DHFR transfected cells and an anticancer compound that inhibits the metabolism of folic acid.
TOKU-E offers three forms of methotrexate: methotrexate (M027), methotrexate sodium (M031), and methotrexate, Evopure® (M091). In aqueous solution, methotrexate sodium is freely soluble. Methotrexate is practically insoluble in water.
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CAS Number
59-05-2
Molecular Formula
C20H22N8O5
Molecular Weight
454.44
Mechanism of Action
In cancerous cells, methotrexate acts as an allosteric inhibitor of dihydrofolate reductase (DHFR), an enzyme involved in the folic acid metabolic pathway. Folic acid is essential in cells because it is required for thymidine and purine synthesis. Methotrexate therefore acts as a nucleic acid synthesis inhibitor.