108321-42-2
C20H40N4O10 · 2 H2SO4
692.71
G418 disulfate, and other aminoglycosides, including kanamycin and neomycin, prevent protein synthesis by blocking the elongation step in prokaryotic and eukaryotic ribosomes.
Mechanism of resistance:
Resistance to G418 sulfate is conferred by the neo gene (neomycin resistant gene) from either Tn5 or Tn601 (903) transposons. Cells successfully transfected with resistance plasmids containing the neo resistance gene can express aminoglycoside 3'-phosphotransferase (APT 3' I or APT 3' II) which covalently modifies G418 to 3-phosphoric G418. 3-phosphoric G418 has negligible potency and has low-affinity for prokaryotic or eukaryotic ribosomes.
2-8°C
2941.90.1010
G418 disulfate is toxic to susceptible prokaryotic and eukaryotic cells including fungi (yeasts and molds), bacteria, mammalian and plant cells.
Powder
White or off-white powder
ED50 Resistant: ≥2500 ug/mL
ED50 Sensitive: ≤400 ug/mL
Carbon: 28.8 - 36.07%
Hydrogen: 5.76 - 7.76%
Nitrogen: 6.72 - 8.41%
Water of hydration: 0 to 6
≤1.0%
≤12.0%
Not less than 720 ug/mg
1mg/mL:280nm <0.015
100mg/mL: 570nm <0.10
(200mg/mL) 4.6 - 6.0
≤1 EU/mg
