G418disulfate,alsoknownasG418sulfate,isroutinelyusedasaselectionantibioticincellculturegeneselectionapplications.G418disulfateisanaminoglycosideantibioticisolatedfrom Micromonosporarhodorangea andiscloselyrelatedtothegentamicins;mostnotably,gentamicinB1.G418isagenericnameofGeneticin.®
G418disulfate50mg/mL(G021)ispreparedasasolutionataconcentrationof50mg/mLinwaterforquickandeasydilutionfactorcalculations.
CASNumber
108321-42-2
MolecularFormula
C20H40N4O10·2H2SO4
MolecularWeight
692.71g/mol
MechanismofAction
G418disulfate,andotheraminoglycosides,includingkanamycinandneomycin,preventproteinsynthesisbyblockingtheelongationstepinprokaryoticandeukaryoticribosomes.Mechanismofresistance:
ResistancetoG418sulfateisconferredbytheneogene(neomycinresistantgene)fromeitherTn5orTn601(903)transposons.Cellssuccessfullytransfectedwithresistanceplasmidscontainingtheneoresistancegenecanexpressaminoglycoside3"-phosphotransferase(APT3"IorAPT3"II)whichcovalentlymodifiesG418to3-phosphoricG418.3-phosphoricG418hasnegligIBLepotencyandhaslow-affinityforprokaryoticoreukaryoticribosomes.
StorageConditions
2-8°C
TariffCode
2941.90.1010
Spectrum
G418disulfateistoxictosusceptibleprokaryoticandeukaryoticcellsincludingfungi(yeastsandmolds),bacteria,mammalianandplantcells.
Form
Solution
Appearance
Clearandcolorlesssolution
Source
Micromonosporarhodorangea
ED50Resistant:Notlessthan2,500µg/mL
ED50Sensitive:Notmorethan400µg/mL
ElementalAnalysis
Carbon:28.80-36.07%
Hydrogen:5.76-7.76%
Nitrogen:6.72-8.41%
WatersofHydration:0to6
Ammonia
Notmorethan1.0%
WaterContent(KarlFisher)
(Powder)Notmorethan12.0%
Potency(onadrybasis)
Notlessthan720µg/mg
Absorbance
280nm(1mg/mL):Notmorethan0.015
570nm(100mg/mL):Notmorethan0.10
pH
4.6-6.0
OpticalRotation
+104°to+121°
Concentration
50mg/mL
