Puromycin is a nucleoside antibiotic isolated from Streptomyces alboniger in the 1950s as an anti-trypanosomal agent with antibiotic activity. Puromycin is used in cell biology to select mammalian cell lines that have been transformed by vectors that express puromycin-N-acetyl-transferase.
Puromycin is soluble in ethanol, methanol, DMF and DMSO and has moderate solubility in water.
CAS Number
53-79-2
Molecular Formula
C22H29N7O5
Molecular Weight
471.5
Mechanism of Action
Puromycin is non-selective, inhibiting RNA by blocking ribosomal translation.
Storage Conditions
-20°C