BlasticidinSHClisapeptidylnucleosideproducedbyseveralspeciesofStreptomycesthatwasfirstisolatedfromS.griseochromogenesin1958.BlasticidinSinhibitsproteinsynthesisandisactiveagainstbacteria,fungi,nematodes,andtumorcells.Thecompoundisusedasaselectionantibioticforbotheukaryoticandprokaryoticcells,andaMarkerforstrainmanipulation.

TOKU-EcarriesthreeformsofBlasticidinS:

• BlasticidinSHCl(B001)
• BlasticidinS(B052)
• BlasticidinSHClSolution(10mg/mlin20mMHEPES)(B006-B007)

BlasticidinSissolubleinwater(5-10mg/ml)andaceticacid.

Thisproductisconsideredadangerousgood.Quantitiesabove1gmaybesubjecttoadditionalshippingfees.

CASNumber

3513-03-9

MolecularFormula

C₁₇H₂₆N₈O₅·HCl

MolecularWeight

458.90

MechanismofAction

BlasticidinSHClinhibitsproteinsynthesisinprokaryoticandeukaryoticcellsbybindingtotheribosomalP-sitewhichstrengthenstRNAbindingandslowsdownandpreventssubsequentpeptidesynthesis.

Mechanismsofresistance

ResistancetoblasticidinSisconferredbybsr,BSD,andblsresistancegenesisolatedfromBacilluscereusK55-S1,Aspergillusterreus,andStreptoverticillumspp,respectively.

Thebsrresistancegeneisa420bpfragmentandencodesa15kDablasticidinSdeaminasewhichcatalyzesthereactionofblasticidinStodeaminohydroxyblasticidinS.DeaminohydroxyblasticidinSisaBIOLOGicallyinactivederivativeofblasticidinSanddoesnotinteractwithorinhibitprokaryoticoreukaryoticribosomes.

Thebsdresistancegeneisa393bpfragmentandalsoencodesablasticidinSdeaminaseenzymewhichcatalyzesasimilarreactiontotheBSRdeaminase.AstudybyKimuraetal.foundthetransfectionfrequencywithbsdtobe80XgreaterthanwithbsrwhenusingFM3Acells.

Theblsgeneresistancegeneencodesanacetyltransferasewhichinteractswithacetyl-coenzymeAandpreventsblasticidinSfrominhibitingproteinsynthesis.

StorageConditions

2-8°C;

TariffCode

2934.99.9000

Spectrum

BlasticidinSHClisbiologicallyactiveagainstsusceptIBLemammalianandprokaryoticcells.