Nitrocefin is a chromogenic cephalosporin used to detect β-lactamases produced by β-lactam resistant bacteria. It does not appear to have antimicrobial properties, but has useful diagnostic properties. It can be used as a reagent in beta-lactamase activity studies. Nitrocefin is soluble in DMSO.
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CAS Number
41906-86-9
Molecular Formula
C21H16N4O8S2
Molecular Weight
516.51
Mechanism of Action
β-lactamases hydrolyze the amide bond between the carbonyl carbon and the nitrogen in the β-lactam ring of Nitrocefin.Intact Nitrocefin is converted to hydrolyzed Nitrocefin under visible light (~ 380-500 nm) and a color change from yellow (max at pH 7.0 = 390 nm) to red (max at pH 7.0 = 486 nm).
Storage Conditions
-20°C; Protect from light; Store with inert gas.
Tariff Code
2934.99.3900
