SpectinomycinSulfateisanaminocyclitolantibioticthatisisolatedfromStreptomycesspectABIlis.SpectinomycinwasdiscoveredinculturebrothbyresearchersattheUpjohnCompanyin1961.SpectinomycinSulfateshowsactivityagainstgram-positivebacteria,gram-negativebacteria,andMycoplasma.

SpectinomycinSulfateisaproteinsynthesisinhibitor.Itactsbyinhibitingproteinsynthesisandelongationbybindingtothebacterial30SribosomalsubunitandinterferingwithpeptidyltRNAtranslocation.Thiseventuallyleadstobacterialcelldeath.

SpectinomyciniscommonlyusedasaselectiveagenttoisolatecellsthatcontainaadAspectinomycinresistancegenes.ItalsohasvarioususesinplantBIOLOGyapplications.

TOKU-Eofferstwoformsofspectinomycin:spectinomycinDiHCl(S006)andSpectinomycinsulfate(S016).Inaqueoussolution,spectinomycinDiHClisfreelysoluble,andspectinomycinsulfateissparinglysoluble(0.150mg/mL).Synonyms:Togamycin,Trobicin,Spectam,Actinospectacin,U-18409E

CASNumber

64058-48-6

MolecularFormula

C₁₄H₂₆N₂O₁₁S•H₂SO₄•4H₂O

MolecularWeight

502.49

MechanismofAction

Theaminocyclitolantibioticspectinomycin,oftenconsideredalongsidetheaminoglycosides,bindsinreversIBLefashion(hencethebacteriostaticactivity)tothe16SrRNAoftheribosomal30Ssubunit.ThereitinterruptsthetranslocationeventthatoccursasthenextcodonofmRNAisalignedwiththeAsiteinreADInessfortheincomingaminoacyl-tRNA.Structuralstudiesrevealthattheantibioticbindstoanareaofthe30Ssubunitknownastheheadregionwhichneedstomoveduringtranslocation.Bindingoftherigidspectinomycinmoleculeappearstopreventthemovementrequiredfortranslocation.

StorageConditions

2-8°C

TariffCode

2941.90.5000

Spectrum

Spectinomycinsulfateisanantibioticthatisactiveagainstavarietyofaerobicgram-negativeandgram-positiveorganismsaswellasMycoplasmaspecies.Spectinomycinisusedclinically,primarilyforitsactivityagainstgram-negativeorganisms;somegram-positiveorganismsmayalsobesusceptibletothisagent.Anaerobicorganismsaregenerallyresistant.Spectinomycinisusuallybacteriostaticattherapeuticdoses.